|
|
|
| Registros recuperados: 55 | |
|
|
| Amoli,Mahsa M.; Amiri,Parvin; Tavakkoly-Bazzaz,Javad; Charmchi,Elham; Hafeziyeh,Jila; Keramatipour,Mohammad; Abiri,Maryam; Ranjbar,Shirin Hasani; Larijani,Bagher. |
| The transcription factor 7-like 2 gene (TCF7L2) rs7903146 T allele is constantly associated with Type 2 diabetes in various populations and ethnic groups. Nevertheless, this has not been observed in two studies involving Arab populations. The aim of the present study was to investigate the association between TCF7L2 rs7903146 in an Iranian population. Type 2 diabetes patients (N = 258) and normal healthy control subjects (N = 168) from the same area, were examined. The ARMS-PCR (Amplification Refractory Mutation System) technique, subsequently validated by direct sequencing, was used for genotyping. Allele and genotype frequencies were significantly different between patients and controls TT vs. CT + CC [p 0.0081 OR 3.4 95%CI (1.27-11.9)] and T vs. C... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: TCF7L2; Gene; Polymorphism; T2DM. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300010 |
| |
|
| |
|
| |
|
| |
|
| |
|
|
| Bacca, Helene; Huvet, Arnaud; Fabioux, Caroline; Daniel, Jean-yves; Delaporte, Maryse; Pouvreau, Stephane; Van Wormhoudt, A; Moal, Jeanne. |
| To investigate the control at the mRNA level of glycogen metabolism in the cupped oyster Crassostrea gigas, we report in the present paper the cloning and characterization of glycogen phosphorylase and synthase cDNAs (Cg-GPH and Cg-GYS, respectively, transcripts of main enzymes for glycogen use and storage), and their first expression profiles depending on oyster tissues and seasons. A strong expression of both genes was observed in the labial palps and the gonad in accordance with specific cells located in both tissues and ability to store glucose. Cg-GPH expression was also found mainly in muscle suggesting ability to use glycogen as readily available glucose to. supply its activity. For seasonal examinations, expression of Cg-GYS and Cg-GPH genes... |
| Tipo: Text |
Palavras-chave: Reproduction; Regulation; Oyster; Glycogen; Expression; Gene; Energy; Crassostrea gigas; Bivalve. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-435.pdf |
| |
|
|
| Bassoi,Manoel Carlos; Flintham,John. |
| Since red alleles (R) of the genes that control grain colour are important for the improvement of preharvest sprouting resistance in wheat and there are three independently inherited loci, on chromosomes 3A, 3B and 3D of hexaploid wheat, it is possible to vary the dosage of dominant alleles in a breeding program. The objective of this work was to evaluate the dosage effect of R genes on preharvest sprouting, in a single seed descent population, named TRL, derived from the cross between Timgalen, white-grained wheat, and RL 4137, red-grained wheat. The study was carried out using sprouting data in ripe ears obtained under artificial conditions in a rainfall simulator over three years. According to the results there is a significant effect on preharvest... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Triticum aestivum; Gene; Allele; Dormancy; Dosage effect. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2005001000006 |
| |
|
| |
|
|
| Biffi,CP; Stefani,LM; Miletti,LC; Matiello,CA; Backes,RG; Almeida,JM; Neves,GB. |
| Isolates of Salmonella sp (104) from poultry samples were isolated and serotyped where eleven were identified as Salmonella Typhimurium (ST). ST isolates were phenotypically tested by disk diffusion and minimum inhibitory concentration (MIC). Four genes related to enrofloxacin (GyrA, GyrB, ParC and ParE), two to gentamicin (AadA and AadB) and two to ceftiofur (BlaCMY-2 and AmpC) resistance were searched by PCR. Our results showed ST resistance to all three antibiotics tested (18.1% for ceftiofur, 45.4% for gentamicin, and 18.1% for enrofloxacin) according to the diffusion test. In the MIC test, the ST isolates showed higher levels of resistance (27.2% for ceftiofur, 54.5% for gentamicin, and 18.2% for enrofloxacin). Three resistance genes out of four... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antimicrobial; Gene; Resistance; Salmonella. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000200013 |
| |
|
| |
|
| |
|
|
| CORDEIRO, M. C. R.. |
| ABSTRACT: Basic concepts and methodologies concerning genetic engineering are presented in this review and is directed to people or professionales not familiarized to it. Moreover, it can be a document to clarity what is genetic engineering, the importance of its contributions to modern agriculture, medicine or livestock research and to support discussions on genetic research ethics. Covers information on structure of nucleic acids, types, functions and main processes such us DNA duplication, transcription and traduction. Important methodologies are covered about DNA/RNA extraction, electrophoresis, PCR, nucleic acid libraries production, cloning, sequencing, gene characterization in connection to main historical events of genetic engineering. It... |
| Tipo: Documentos (INFOTECA-E) |
Palavras-chave: Ácido Nucléico; Biologia Molecular; Biotecnologia; Clonagem; DNA; Engenharia Genética; Gene; Marcador Genético; RNA; Organismo Transgênico.. |
| Ano: 2003 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/568132 |
| |
|
| |
|
|
| Delsert, Claude; Cancela Da Fonseca, Leonor. |
| The main objective of this project was to develop the tools for the in-depth understanding of mollusc biology and pathology. Such a goal has been reached in other biological systems through the development of molecular biology and cell culture. Indeed, development of a mollusc cell culture system is an essential step to approach many of the problems related to mollusc pathology, in particular to use molecular biology tools to study viral infection of these organisms. No continous cell line is available from bivalves and while cell cultures have been obtained from these organisms they are difficult to develop and labor intensive, survival cultures being maintained only for a few weeks. Our goal has been to study in detail many of the parameters involved in... |
| Tipo: Text |
Palavras-chave: Pathology; Mussel-specific expression vectors; Cell culture; Mytilus galloprovincialis; Gene; Genetic marker. |
| Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/00044/15540/12917.pdf |
| |
|
|
| Diaz Almela, F; Boudry, Pierre; Launey, Sophie; Bonhomme, Francois; Lapegue, Sylvie. |
| The geographical structure of 15 natural populations of the flat oyster (Ostrea edulis L.) was assessed by single-strand conformation polymorphism (SSCP) of a 313-base-pair (bp) fragment of the mitochondrial 12S-rRNA gene. Fourteen haplotypes were observed, with one being dominant in the Mediterranean samples and another one in the Atlantic populations. The geographically extreme populations sampled in Norway and the Black Sea appeared differentiated by exhibiting the dominance of a third group of haplotypes. The results were compared to available microsatellite data at five loci. The Atlantic/Mediterranean differentiation pattern was qualitatively the same with both types of markers, confirming an isolation-by-distance pattern. The average mitochondrial... |
| Tipo: Text |
Palavras-chave: Ostrea edulis; Polymorphism; Flat oyster; Population; Gene. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-680.pdf |
| |
|
| |
|
|
| El-Hani,Charbel Niño. |
| Challenges to the gene concept have shown the difficulty of preserving the classical molecular concept, according to which a gene is a stretch of DNA encoding a functional product (polypeptide or RNA). The main difficulties are related to the overlaying of the Mendelian idea of the gene as a unit: the interpretation of genes as structural and/or functional units in the genome is challenged by evidence showing the complexity and diversity of genomic organization. This paper discusses the difficulties faced by the classical molecular concept and addresses alternatives to it. Among the alternatives, it considers distinctions between different gene concepts, such as that between the molecular and the evolutionary gene, or between gene-P (the gene as... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gene; Classical molecular gene concept; Mendelian gene; Genomic complexity. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300001 |
| |
|
|
| Fagundes,RBS; Picoli,EAT; Lana,UGP; Valicente,FH. |
| Bacillus thuringiensis harbors genes encoding Cry proteins found in chromosomes or plasmids of different sizes (4-150 Mb). Although the smaller plasmids are more abundant in B. thuringiensis, their specific function is unknown. As for the megaplasmids, their main recognized function is to harbor cry genes, although the sequencing of some of these plasmids indicates the occurrence of other important genes. This work used a new protocol for practical and rapid extraction of plasmid DNA in order to characterize the plasmid patterns of Brazilian strains belonging to Embrapa Milho e Sorgo research center B. thuringiensis bank. We tried to further assess the relationship of plasmid patterns with strains belonging to the same serovars and strains causing 100% and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacterium; Cry protein; Gene; Megaplasmid. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2011000500012 |
| |
|
| |
|
|
| Fleury, Elodie; Huvet, Arnaud. |
| Summer mortality of Crassostrea gigas is the result of a complex interaction between oysters, their environment, and pathogens. A high heritability was estimated for resistance to summer mortality, which provided an opportunity to develop lines of oysters that were resistant (R) or susceptible (S) to summer mortality. Previous genome-wide expression profiling study of R and S oyster gonads highlighted reproduction and antioxidant defense as constitutive pathways that operate differentially between these two lines. Here, we show that signaling in innate immunity also operates differentially between these lines, and we hypothesize that this is at the main determinant of their difference in survival in the field. A reanalysis of our published microarray data... |
| Tipo: Text |
Palavras-chave: Mollusca; Crassostrea gigas; Gene; Immunity; CDNA microarray; Summer mortality. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00083/19423/17213.pdf |
| |
| Registros recuperados: 55 | |
|
|
|
